I am currently working towards my PhD in Zoology at Stellenbosch University, South Africa. I am describing coastal fish biodiversity in the region using an environmental DNA (eDNA) metabarcoding approach.
Currently: I am researching the biodiversity of fishes across the South African coastline. Interestingly, these fishes (and all organisms) shed DNA into the environment as they move and exist in their habitat, and the DNA that they shed can come from things like their scales, their urine and their faeces. The oceans are therefore a big soup containing loads of amazing creatures, and also the free-floating DNA of these creatures!
Using an increasingly powerful method called environmental DNA metabarcoding, I am capturing the free-DNA (called environmental DNA or eDNA) and matching this DNA to known DNA sequences to identify which fishes are present along different areas of the South African coastline. It involves me travelling to many different shores, from rocky and sandy shores to seagrass meadows and mangrove forests, and collecting seawater. I filter this seawater to capture any DNA that is floating in the water, and bring it back to the lab for DNA extractions, followed by sequencing, and then computer-based processing and analyses (this whole process is known as DNA metabarcoding).
I am lucky to spend time in the field collecting water samples, but the really exciting stuff happens once we get back to the lab and process the data, which contains species lists for all the places that we visited! This method allows us to describe fish biodiversity without ever having to actually see the fishes that we are trying to describe. Most fish biodiversity estimates in the region rely on data from fish that are caught on commercial fishing vessels, which is very selective depending on the type of fishing gear you use and therefore not representative of what fish are actually occurring in those waters. Even using visual surveys (e.g. by SCUBA diving and recording the species that you see) can be biased towards larger species, surveys can often mis-identify cryptic or similar species, and require lots of taxonomic expertise.
We are therefore describing fish biodiversity across South Africa using the DNA metabarcoding approach instead, giving us rapid, cost-effective and more up-to-date information on fishes in the region. This type of data is vital for appropriately managing these important coastal resources.
Previously: My journey as a marine biologist started at the University of Manchester where I began my BSc in Zoology, before transferring to Bangor University for a BSc Joint Honours in Marine Biology and Zoology. I graduated from Bangor with an MSci Master of Marine Biology with International Experience. I’ve worked on various marine systems and organisms, from the effects of bio-acidification on shark development (University of Manchester), gene expression (AWI, Germany) and echinoculture (Bangor University) to mangrove biodiversity (Kenya Marine and Fisheries Research Institute) and now coastal fish biodiversity in South Africa (Stellenbosch University).
News and Media: I enjoy creating media, some of which you can see by clicking here, and there are various news articles about our research that you can access here. I am also active on Twitter @zoologymolly, often tweeting with our #SAeDNA hashtag with updates on our eDNA work here in South Africa.
Feel free to contact me via twitter, or by email at firstname.lastname@example.org.
Blog: Visit my blog here